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Part:BBa_K2592006:Design

Designed by: Janice Law   Group: iGEM18_Hong_Kong_HKU   (2018-09-23)


Strand 2 for in-vivo synthesis of Nano Drug Carrier-AS1411 (NDC-as)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N.A.



Source

This part is modified from the design published in 'Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Elbaz et al. Nat Commun. 2016 Apr 19;7:11179.'. AS1411 aptamer sequence is from 'Antiproliferative Activity of G-rich Oligonucleotides Correlates with Protein Binding. Bates et al. The Journal of Biological Chemistry. 1999 Sep 10;274: 26369-26377.'.

References